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| Simon-colin, Christelle; Alain, Karine; Colin, Sebastien; Cozien, Joelle; Costa, B; Guezennec, Jean; Raguenes, Gerard. |
| Aims: The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. Methods and Results: This micro-organism, on the basis of the phenotypical features and genotypic investigations can be clearly assigned to the Pseudomonas genus and the name of Pseudomonas guezennei is proposed. Optimal growth occurs between 33 and 37 degrees C, at a pH between 6 4 and 7 1 and at ionic strength of 15 g 1(-1) of sea salts. The G+C content of DNA is 63.2%. Under laboratory conditions, this bacterium produced a novel, medium-chain-length PHA, mainly composed of... |
| Tipo: Text |
Palavras-chave: Pseudomonas; Polyhydroxyalkanaote; Microbial mat; Kopara. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3858.pdf |
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| Simon-colin, Christelle; Raguenes, Gerard; Crassous, Philippe; Moppert, X; Guezennec, Jean. |
| Pseudomonasguezennei biovar. tikehau was isolated from a microbial mat on the atoll of Tikehau in French Polynesia, and is able to synthesize medium chain length poly-beta-hydroxyalkanaote copolymers when grown on coprah oil. A two-step cultivation process was used and the biosynthesis of PHAs was followed along 52 h by regular culture sampling. The polyester was purified from freeze-dried cells and analysed by nuclear magnetic resonance (NMR), Fourier transform infra red (FTIR), and gas chromatography mass spectrometries. The copolyester produced by P. guezennei biovar. tikehau from coprah oil mainly consisted of saturated monomers, i.e. 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), and the monomeric composition of the polyester did not change... |
| Tipo: Text |
Palavras-chave: Polyhydroxyalkanaote; PHAs; Pseudomonas; "kopara"; Microbial mat. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6119.pdf |
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